Performance

technical_services_operation

Technical services and operations to get performance out of the equipment.

Support for several microscopy techniques, including, but not limited to:

  • Transmitted light microscopy (bright field, dark field, phase contrast, polarization and DIC/Nomarski)
  • Cytoplasmic and nuclear microinjection
  • Live sample imaging
  • Multiple fluorescent labeling
  • Fluorescence microscopy (wide-field, laser scanning confocal, spinning disk confocal, FRAP, FRET, FLIM, FCS, TIRF, multi-photon and structured illumination)
  • Super-resolution techniques (SIM, PALM/DSTORM, STED)
  • Laser microdissection

Expertise with upright and inverted microscopy systems from Zeiss, Olympus, Leica and Nikon.

Competent operation of sophisticated systems.  Maximize sample protection, optimize equipment performance and rapidly acquire data quantitatively. No need to learn yourself, and to worry about artefacts and equipment breakdown resulting from an insufficient mastering of the system.

Management of light microscopy systems and quality control plans to ensure usage at full potential.

 

Microscopy Equipment Quality Control Plans

General inspection and cleaning of the workstation:

  • Microscope, including stage (especially oil and spills), computer, table, etc
  • Air table (level, pressure and responsiveness)

Transmitted light path:

  • Condenser alignment
  • Eyepieces
  • Alignment of differential interference contrast (DIC) elements and/or phase contrast rings 

Objectives:

  • Qualitative evaluation (dust, dirt, scratches, etc)
  • Visual detection of the secondary ring of the Airy diffraction pattern
  • Visual evaluation of objective’s planapochromatic performance
  • Cleaning (oil, spills, mounting media, etc)
  • Cleaning kit
  • Quantitative evaluation (resolution and shape, spherical and chromatic aberrations)

Fluorescence:

  • Alignment and even illumination (bulb replacement when needed)
  • Inspection and cleaning (entry/exit lens, filter cubes, ND filters)
  • Widefield light source intensity measurement
  • Laser performance (intensity, stability, alignment, illumination uniformity, mode-locking)
  • Light through put efficiency (photo-electron conversion factor)
  • Filter cube performance (color registration, chromatic aberrations)
  • Dichroic mirrors and emission filters performance (color registration, chromatic aberrations)

Detector:

  • Image distortion evaluation
  • Performance (offset and noise)
  • Pinhole alignment
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